PChem Teaching Lab | Tables and Charts

BUFFER STOCK SOLUTIONS

No. Stock A Stock B Buffer Solution Composition

1 0.1M Glycine & 0.1M NaCl 0.1M HCl x parts A & (100-x) parts B

2 0.1M di-Sodium citrate 0.1M HCl x parts A & (100-x) parts B

3 0.1M Potassium hydrogen phthalate 0.1M HCl 50ml A & x ml B; make up to 100ml

4 0.0667M Potassium dihydrogen phosphate 0.0667M di-Sodium hydrogen phosphate x parts A & (100-x) parts B

5 0.1M 5,5-Diethylbarbituric acid sodium salt 0.1M HCl x parts A & (100-x) parts B

6 0.05M Borax solution 0.1M HCl x parts A & (100-x) parts B

7 0.1M Citric acid 0.2M di-Sodium hydrogen phosphate x parts A & (100-x) parts B

8 0.1M Sodium acetate 0.1M Acetic acid x parts A & (100-x) parts B

9 0.2M Imidazole 0.1M HCl 25ml A & x ml B; make up to 100ml

10 0.2M Tris(hydroxymethyl)aminomethane 0.1M HCl 25ml A & x ml B; make up to 100ml

11 0.1M Sodium carbonate 0.1M Sodium hydrogen carbonate x parts A & (100-x) parts B

BUFFER SOLUTIONS

Note: The numbers in the table below represent the value of x (in ml) to
be used in the "Buffer Solution Composition" column of the previous table.

pH No.1 No.2 No.3 No.4 No.5 No.6 No.7 No.8 No.9 No.10 No.11

1.2 11.1 9.0

1.4 26.4 17.9

1.6 36.2 23.6

1.8 43.9 27.6

2.0 50.7 30.2

2.2 56.5 32.2 98.8

2.4 62.3 34.1 41.0 94.5

2.6 68.4 36.0 34.3 90.0

2.8 74.7 37.9 27.8 85.1

3.0 81.0 39.9 21.6 80.3

3.2 86.2 42.1 15.9 76.0

3.4 90.3 44.8 10.9 72.0

3.6 47.8 6.7 68.4

3.8 51.2 3.3 65.1 10.9

4.0 55.1 0.0 62.0 16.6

4.2 60.0 59.1 23.9

4.4 66.4 56.4 33.5

4.6 74.9 53.7 44.9

4.8 85.6 51.2 56.6

5.0 100.0 99.2 49.0 67.8

5.2 98.4 46.9 76.8

5.4 97.3 44.7 84.0

5.6 95.5 42.2 89.3

5.8 92.8 40.0

6.0 88.9 37.4

6.2 83.0 34.5 43.4

6.4 75.4 31.4 40.4

6.6 65.3 27.9 36.5

6.8 53.4 23.5 31.4

7.0 41.3 53.3 19.0 25.4

7.2 29.6 55.0 13.8 19.6 44.7

7.4 19.7 57.6 9.8 14.6 42.0

7.6 12.8 60.8 6.8 10.2 39.3

7.8 7.4 65.2 53.0 4.6 6.6 33.7

8.0 3.7 70.6 55.4 27.9

8.2 75.6 58.0 22.9

8.4 81.2 62.1 17.3

8.6 86.2 66.9 13.0

8.8 90.1 73.6 8.8

9.0 93.2 83.5 5.3

9.2 95.6 10.0

9.4 18.4

9.6 29.3

9.8 42.0

10.0 53.4

10.2 63.7

10.4 73.1

10.6 81.2

10.8 87.9

pH No.1 No.2 No.3 No.4 No.5 No.6 No.7 No.8 No.9 No.10 No.11

Charts & Tables

BUFFER STOCK SOLUTIONS
No.	Stock A	Stock B	Buffer Solution Composition
1	0.1M Glycine & 0.1M NaCl	0.1M HCl	x parts A & (100-x) parts B
2	0.1M di-Sodium citrate	0.1M HCl	x parts A & (100-x) parts B
3	0.1M Potassium hydrogen phthalate	0.1M HCl	50ml A & x ml B; make up to 100ml
4	0.0667M Potassium dihydrogen phosphate	0.0667M di-Sodium hydrogen phosphate	x parts A & (100-x) parts B
5	0.1M 5,5-Diethylbarbituric acid sodium salt	0.1M HCl	x parts A & (100-x) parts B
6	0.05M Borax solution	0.1M HCl	x parts A & (100-x) parts B
7	0.1M Citric acid	0.2M di-Sodium hydrogen phosphate	x parts A & (100-x) parts B
8	0.1M Sodium acetate	0.1M Acetic acid	x parts A & (100-x) parts B
9	0.2M Imidazole	0.1M HCl	25ml A & x ml B; make up to 100ml
10	0.2M Tris(hydroxymethyl)aminomethane	0.1M HCl	25ml A & x ml B; make up to 100ml
11	0.1M Sodium carbonate	0.1M Sodium hydrogen carbonate	x parts A & (100-x) parts B

BUFFER SOLUTIONS
Note: The numbers in the table below represent the value of x (in ml) to be used in the "Buffer Solution Composition" column of the previous table.
pH	No.1	No.2	No.3	No.4	No.5	No.6	No.7	No.8	No.9	No.10	No.11
1.2	11.1	9.0
1.4	26.4	17.9
1.6	36.2	23.6
1.8	43.9	27.6
2.0	50.7	30.2
2.2	56.5	32.2					98.8
2.4	62.3	34.1	41.0				94.5
2.6	68.4	36.0	34.3				90.0
2.8	74.7	37.9	27.8				85.1
3.0	81.0	39.9	21.6				80.3
3.2	86.2	42.1	15.9				76.0
3.4	90.3	44.8	10.9				72.0
3.6		47.8	6.7				68.4
3.8		51.2	3.3				65.1	10.9
4.0		55.1	0.0				62.0	16.6
4.2		60.0					59.1	23.9
4.4		66.4					56.4	33.5
4.6		74.9					53.7	44.9
4.8		85.6					51.2	56.6
5.0		100.0		99.2			49.0	67.8
5.2				98.4			46.9	76.8
5.4				97.3			44.7	84.0
5.6				95.5			42.2	89.3
5.8				92.8			40.0
6.0				88.9			37.4
6.2				83.0			34.5		43.4
6.4				75.4			31.4		40.4
6.6				65.3			27.9		36.5
6.8				53.4			23.5		31.4
7.0				41.3	53.3		19.0		25.4
7.2				29.6	55.0		13.8		19.6	44.7
7.4				19.7	57.6		9.8		14.6	42.0
7.6				12.8	60.8		6.8		10.2	39.3
7.8				7.4	65.2	53.0	4.6		6.6	33.7
8.0				3.7	70.6	55.4				27.9
8.2					75.6	58.0				22.9
8.4					81.2	62.1				17.3
8.6					86.2	66.9				13.0
8.8					90.1	73.6				8.8
9.0					93.2	83.5				5.3
9.2						95.6					10.0
9.4											18.4
9.6											29.3
9.8											42.0
10.0											53.4
10.2											63.7
10.4											73.1
10.6											81.2
10.8											87.9
pH	No.1	No.2	No.3	No.4	No.5	No.6	No.7	No.8	No.9	No.10	No.11